1- Human and Animal Cell Culture: 
• Cell culture laboratories and instructions of working in cell culture room
• An introduction to cell lines and morphological characteristics of cells in media 
• Different cell culture media and their characteristics 
• Fundamentals of cell passage protocols, freezing and defreezing of cell lines
• Quality control techniques and microbial contamination of cell cultures
• Principles of cell banks
 
2- Measuring the Toxicity Effect of Substances and Checking Cell Proliferation and Viability by MTT Method
 
• Methods of measuring the degree of toxicity effect of substances and study of cell proliferation
• General principles of Cell Culture
• Measuring optimum cell counts and constructing standard curve
• Method of performing MTT test and Data analysis
 
Prerequisite: The participant should be familiar with Cell Culture
 
3- Flow Cytometry Workshop: Principles, Data Analysis and Application in Biological Science
 
• Mechanism and manual of working with Flow Cytometry apparatus and settings
• Selecting fluorochrome and panel design 
• Simultaneous application of colors, compensation concepts
• Controlling isotype antibody and method of selection
• Application of Flow Cytometry (analysis of cell cycle, appaptosis and cell proliferation)
• Sample preparation 
• Sample assessment using Flow Cytometry apparatus 
• Working with device software and Flowjo software
 
4- In Vitro Maturation of Oocytes in Mice (IVM in Reproductive Biology Field) 
• A review on whole procedure 
• General principles and safety of working with strategic laboratory facilities 
• General principles of working with laboratory animals
• Methods of preparing culture media and preparations for collecting oocytes ant its maturation
• Methods of stimulating ovulation of female mice by hormone injection 
• Surgery of female mice and collecting oocytes from ovary 
• In vitro maturation of oocytes and method of diagnosis
 
5- Workshop on Specialized Surgeries of Reproductive Tissues on Small Laboratory Mice (Reproductive Science)
• Basics of general surgery of the small laboratory mice
• Specialized surgeries of the reproductive system of the male mice for sperm extraction
• Specialized surgeries of the reproductive system of the female mice for oocyte and fetus extraction
• Training on the vasectomy surgery method in male mice to produce infertile mice in various research project and use in embryo transfer project 
• Surgical training related to embryo transfer in the blastocyst stage to the uterus of a female mouse 
 
6- Principles and Methods of Designing and Preparing General and Specialized Culture Media for Cells, Tissues, Gamets and Embryos
• Introduction and training on precise working and methods of calibrating specialized equipment of preparing culture media 
• Training on more important culture media protocols, methods of preparation, sterilization and preservation
• Methods of quality control and validation of prepared media in laboratory 
• How to use culture media 
 
7- Safety Principles and Methods of Working with Practical and Strategic Facilities of Research Laboratories
• Principles of GLP
• Biosafety principles
• Methods of working with laboratory animals 
• Principles of working with strategic laboratory equipment
 
8- Preliminary Techniques of Embryological Research on Small Laboratory Mice
• Ethology of laboratory mice
• Physiology and anatomy of laboratory mice
• Methods of restraining and handling animals
• Determination of drug doses and anesthesia
• Methods of general surgeries on mice
• Specialized surgeries of the reproductive system in male and female mice
 
 
9- Principles of Clinical Cytogenetic
• Naming and classification of human chromosomes 
• An introduction to genetic counseling 
• Genetic counseling and inheritance pattern of hereditary diseases
• Genetic diagnosis before implantation and birth
• Mechanisms of structural and numerical changes of chromosomes 
• Lymphocyte cultivation, harvesting, banding and preparing slides
• Microscopic examinations and analysis of results
 
10- Prevention and Detection of Mycoplasma Contamination in Cell Culture
• Getting to know Mycoplasma
• Risks for cell cultures
• Importance of tests for contamination detection
• Equipment, safety considerations, preventing contamination and methods of cultivating in specific microbial media 
• PCR methods, staining DNA and eradicating 
• Direct culture on specific microbial media
 
11- Production of Immortalized Lymphoblastoid Cell Lines Using Epstein-Barr Virus (EBV)
• Immortalization, definition of basic concepts and requirements
• Methods of immortalization
• B lymphocyte immortalization by EBV
• Material and methods
• Good cell banking practice
 
12- Magnetic Cell Separation Method
• Various methods of cell separation and their application 
• Various methods of magnetic cell separation 
• Practical cell separation using manual magnetic method and concentration gradients